Bpsbioscience/PDE3A, (669-end), His-GST-tags/60032/5 µg
商品编号：
60032
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:82kDa
Genbank#:NM_000921
Tag(s):N-terminalHis-GST-tags
a.a:669–1141(end)
Description:
HumanPDE3A(GenBankAccessionNo.NM_000921),a.a.669-1141(end)withN-terminalHis-GST-tag,MW=82kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q14432
Synonym(s):
PDE3A,phosphodiesterase3A
SpecificActivity:
≥4000pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE3AAssayKit,Cat.#60330.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE3Aat37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glyceroland3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Hung,S.H.,etal.J.Biol.Chem.281(39),29236-29244(2006)
2.Hambleton,R.,etal.J.Biol.Chem.280(47),39168-39174(2005)
ApplicationReference(s):
1.CharacterizationofthebindingpropertiesofT-773asaPETrADIoligandforphosphodiesterase10A(2015)
2.AnalysisofsubstratespecificityandkineticsofcyclicnucleotidephosphodiesteraseswithN"-methylanthraniloyl-substitutedpurineandpyrimidine3",5"-cyclicnucleotidesbyfluorescencespectrometry(2013)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
