Bpsbioscience/PDE3A (Mouse), GST-Tag/60036/5 µg
商品编号:
60036
品牌:
bpsbioscience
市场价:
¥8800.00
美元价:
5280.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Mouse
HostSpecies:Sf9insectcells
MW:100kDa
Genbank#:NM_018779
Tag(s):N-terminalGST-tag
a.a:484-1141
Description:
MousePDE3A(GenBankAccessionNo.NM_018779),aminoacids484-1141end),withN-terminalGSTtag,MW=100kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
Synonym(s):
PDE3A,phosphodiesterase3A,PDE3
SpecificActivity:
≥280pmol/min/µg
AssayConditions:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3′,5′-cAMPto5′-AMPperminat37°C.Assaycondition:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,andserialdilutionsofMousePDE3Aat37ºCfor20min.Quantifiedby5′nucleotidasecleavingthe5′-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween20,20%glycerol,18mMGlutathione
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Hung,S.H.,etal.J.Biol.Chem.281(39),29236-29244(2006)
2.Hambleton,R.,etal.J.Biol.Chem.280(47),39168-39174(2005)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。