Bpsbioscience/PDE1B，GST标签/60011/10µg
商品编号：
60011
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:87kDa
Genbank#:NM_000924
Tag(s):N-terminalGST-tag
a.a:fulllength
Description:
HumanPDE1B(GenBankAccessionNo.NM_000924),fulllengthwithN-terminalGST-tag,MW=87kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q01064
Synonym(s):
PDE1B,phosphodiesterase1B
SpecificActivity:
≥7600pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5?-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE1BAssayKit,Cat.#60311.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE1Bat37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,380mMNaCl,1mMDTT,10%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.BenderA.T.andBeavoJ.A.Proc.Natl.Acad.Sci.U.S.A.103(2),460-465(2006).
2.BenderA.T.etal.,Proc.Natl.Acad.Sci.U.S.A.102(2),497-502(2005).
ApplicationReference(s):
AnalysisofsubstratespecificityandkineticsofcyclicnucleotidephosphodiesteraseswithN"-methylanthraniloyl-substitutedpurineandpyrimidine3",5"-cyclicnucleotidesbyfluorescencespectrometry(2013)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
