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当前位置: 首页 > 产品中心 > cytokine > Bpsbioscience/PDE1A, GST-tag/60010/10 µg
商品详细Bpsbioscience/PDE1A, GST-tag/60010/10 µg
Bpsbioscience/PDE1A, GST-tag/60010/10 µg
Bpsbioscience/PDE1A, GST-tag/60010/10 µg
商品编号: 60010
品牌: bpsbioscience
市场价: ¥8800.00
美元价: 5280.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞因子
公司分类: cytokine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:88.3kDa
Genbank#:NM_005019
Tag(s):N-terminalGST-tag
a.a:fulllength
Description:
HumanPDE1A(GenBankAccessionNo.NM_005019),fulllengthwithN-terminalGST-tag,MW=88.3kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
P54750
Synonym(s):
PDE1A,phosphodiesterase1A
SpecificActivity:
≥43,000pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE1AAssayKit,Cat.#60310.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE1Aat37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):

1.Nagel,D.J.,etal.,Circ.Res.98(6),777-784(2006).
2.Goraya,T.A..,etal.,J.Biol.Chem.279(39),40494-40504(2004).

ApplicationReference(s):
1. PDE1AinhibitionelicitscGMP-dependentrelaxationofratmesentericarteries(2017)
2. DiscoveryofaNovelSeriesofPyrazolo[1,5-a]pyrimidine-BasedPhosphodiesterase2AInhibitorsStructurallyDifferentfromN-((1S)-1-(3-Fluoro-4-(trifluoromethoxy)phenyl)-2-methoxyethyl)-7-methoxy-2-oxo-2,3-dihydropyrido[2,3-b]pyrazine-4(1H)-carboxamide(TAK-915),fortheTreatmentofCognitiveDisorders (2017)
3.CharacterizationofthebindingpropertiesofT-773asaPETrADIoligandforphosphodiesterase10A(2015)
4.Geneticdeletionandpharmacologicalinhibitionofphosphodiesterase10Aprotectsmicefromdiet-inducedobesityandinsulinresistance(2014)
5.Thenovelphosphodiesterase10AinhibitorTHPP-1hasantipsychotic-likeeffectsinratandimprovescognitioninratandrhesusmonkey(2013)

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。