Bpsbioscience/PDE11A, GST-Tag (Mouse)/60064/5 µg
商品编号：
60064
品牌：
bpsbioscience
市场价：
¥8900.00
美元价：
5340.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Mouse
Host Species: Sf9 insect cells
MW: 131 kDa
Genbank #: NM_001081033
Tag(s): N-terminal GST-tag
a.a: 2-933(end)
Description:
Mouse PDE11A (GenBank Accession No. NM_001081033), a.a. 2-933(end) with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Synonym(s):
PDE11, phosphodiesterase 11A
Specific Activity:
≥ 1,560 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.
Assay Conditions:
10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE11A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM Glutathione, 10% glycerol, and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Fawcett, L. et al., PNAS. 97(7), 3207-3707 (2000).
2. Horvath, A. et al., Nature Genetics. 38, 794-800 (2006).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
