Bpsbioscience/鼠标PDE5A1，GST标签/60051/5µg
商品编号：
60051
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Mouse
Host Species: Sf9 insect cells
MW: 124 kDa
Genbank #: NM_153422
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Mouse PDE5A (GenBank Accession No. NM_153422), full length, with N-terminal GST-tag, MW=124 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q8CG03
Synonym(s):
PDE5A, phosphodiesterase 5A
Specific Activity:
≥ 4630 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cGMP to 5'-GMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl,
pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 0.1 mg/ml
BSA, 200 µM cGMP, 2.5 kU 5'-nucleotidase, and
serial dilutions of mPDE5A at 37°C for 20 min.
Quantified by 5'- nucleotidase cleaving the
5' -GMP product and releasing the
phosphate group which is detected by
Malachite Green Reagent.
Formulation:
45 mM Tris-HCl, pH 8.0,
124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 18
mM glutathione, 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Das, A. et al., J. Biol. Chem. 280 (13), 12944-12955 (2005).
2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).
2. Champion, H.C.et al., Proc. Natl. Acad. Sci. U.S.A. 102 (5), 1661-1666 (2005).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
