Bpsbioscience/涡轮核酸酶/50310/50000单位
商品编号：
50310
品牌：
bpsbioscience
市场价：
¥5800.00
美元价：
3480.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Serratiamarcescens
HostSpecies:E.coli
MW:27kDa
Description:
Ultra-pureversionofBenzonase.Nonspecificendonuclease,hydrolyzesbothsingle-anddouble-strandednucleicacids(DNAandRNA)to5’-phosphorylatedoligonucleotidesof1-4basesinlength.MW=27kDa(homodimer).ExpressedinE.coli.Nodetectableproteaseactivity.TurboNucleasecanbeusedto
•reduceviscosityofcelllysates
•reducebackpressureofcolumnloADIng
•removenucleicacidcontaminationfromsamplepreparations
•reducenucleicacidcontaminationofNicolumnpurification
•reducesmearinginSDS-PAGEwhenusedwith10%SDSorGelLoadingDye
•makewholecelllysates
•reduceorpreventclumpingofconcentratedcellsandthawedcells
•replacecrudeDNaseIinmanyapplications
•reduceviscosityofcelllysates
•reducebackpressureofcolumnloADIng
•removenucleicacidcontaminationfromsamplepreparations
•reducenucleicacidcontaminationofNicolumnpurification
•reducesmearinginSDS-PAGEwhenusedwith10%SDSorGelLoadingDye
•makewholecelllysates
•reduceorpreventclumpingofconcentratedcellsandthawedcells
•replacecrudeDNaseIinmanyapplications
Synonym(s):
Benzonase
Purity:
≥99%
SuppliedAs:
250units/μlin50mMTris-HCl,pH8.0,50mMNaCl,5mMMgCl2and50%glycerol
EndotoxinLevel:
Totalendotoxinlevelis<0.25EU/1,000unitsofTurboNucleaseasdeterminedbytheLALGel-ClotAssay.Noproteaseactivitywasdetected.
SpecificActivity:
≥1.3x106units/mg(>3x106Kunitzunits/mg)
UnitDefinition:
Oneunitconverts1OD260ofsalmonspermDNAintoacidsolublenucleotidesin30minutesat37°C
AssayConditions:
Reactionbufferof50mMTris-HCl,pH8.0and1mMMgCl2.
Instructionsforuse:
Toreduceviscosityofcelllysate,10-500unitsofTurboNucleasecanbeusedforeachgramofcellpaste.Generally,addingTurboNucleasetocelllysateat25U/mlissufficienttoreducelysateviscosity.Theefficiencyofviscosityreductionmayvarywithbuffers,celltypes,andcelllysismethodsused.Duetoitshighspecificactivity,thetotalamountTurboNucleaseaddedislessthan0.1μg/mloflysateandwillnotcomplicateanydownstreamprocesses.
Storage/StABIlity:
>6monthsat–20°C.Retains>80%activityafterstorageatRTforover65hours.
Application(s):
Idealtodigestnucleicacidsandtoreduceviscosityduringproteinpurificationandsamplepreparation.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Miscellaneous
ProductType:
Enzyme
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
