BPS科学/快速PCR™；无活性细胞克隆试剂盒/20070/10反应
商品编号：
20070
品牌：
bpsbioscience
市场价：
¥4000.00
美元价：
2400.00
产品分类：
常规细胞株
公司分类：
Routine_cell_strain
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
TheQuickPCR™CloningKitisasimpleandhighlyefficientmethodtoinsertanygeneorDNAfragmentintoavector,withouttime-consumingligationsteps.MultiplePCRinsertscanbeaddedinasinglestep.Theprocedureiseasy:aprimersethomologoustothegeneofinterestandthevectorareusedtoamplifyaPCRproduct.ThispurifiedPCRproductisincubatedwithrestrictionenzyme-digestedvectorandReagentAoftheQuickPCR™CloningKit.Followingabrief(5minute)incubationwithReagentB,theannealedvectorisreadyfortransformation.
Description:
TheBPSBioscienceQuickPCR™cloningkitallowsfortheinsertionofmultipleDNAfragmentsintothedesiredvectorinasinglereaction.Noligationstepisrequired,savingtimeandeffort.Themethodisfast,simple,andefficient.GofromPCRproducttotransformingcellsinunderanhour!SimplymixthepurifiedPCRproductwiththevectorina1:1molarratio(TotalDNA:Vector)andfollowtheprotocol.Efficienciesof>90%areroutinelyobtained.Availablewithorwithoutcompetentcellsforevengreaterefficiencyandflexibility.
SuppliedAs:
Components:
ReagentA
ReagentB
BufferA
10XBSA(0.1%w/v)
ReagentA
ReagentB
BufferA
10XBSA(0.1%w/v)
Efficiency:
Efficienciesof>90%aretypical.
Format:
assaykit
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:
Kitcomponentsmaybestoredat-20°Corbelow.Avoidfreeze/thawcycles.Uponfirstthaw,storeallreagentsinsingleusealiquots.Stableforatleast6monthsfromdateofreceipt,whenstoredasdirected.
Application(s):
KitisdesignedforcloninganygeneorDNAfragmentintoanyvectorinaquick,single-stepreaction.Canbeusedwithmultipleinsertsinasinglereaction.Alsosuitableforfixingoraddingpointmutations,insertions,ordeletions.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Mol.Biol.Reag./Cloning
ProductType:
PCRKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
