Bpsbioscience/高效活性细胞/20000/20 x 50µl
商品编号：
20000
品牌：
bpsbioscience
市场价：
¥6500.00
美元价：
3900.00
产品分类：
常规细胞株
公司分类：
Routine_cell_strain
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
Quickgrow™E.coliisspecificallyselectedforitsfastgrowthproperties.UnlikesomeothercommercialE.colistrains,whichtakeatleast14hrsforthecoloniestogrowlargeenoughforpicking,Quickgrow™E.colicangrowtopickablecoloniesonLBplateswithin9hours.Quickgrow™E.coliprovidealpha-complementationofthebeta-galactosidasegeneforcolorselection.Theymaybeusedforproducingsingle-strandedDNAfromM13orphagemidandarealsousefulforinducIBLeproteinexpressionofthosegenesunderLacUV5promotercontrol.ThegenotypeisF"proA+B+lacIqD(lacZ)M15/D(lac-proAB)glnVthi-1D(hsdS-mcrB)5.
SuppliedAs:
Components:
20vials(50µleach)Quickgrow™E.colicells,~1x109cells/vial)
10µlpUC18controlDNA(0.1ng/µl)
10mlSOCmedium
20vials(50µleach)Quickgrow™E.colicells,~1x109cells/vial)
10µlpUC18controlDNA(0.1ng/µl)
10mlSOCmedium
Efficiency:
1.0x109CPU(coloniesperµgpUC18DNA)
AssayConditions:
Transformationsareperformedwith50aliquotsofcellsand2ulofpUC18controlplasmid(10pg/ul)followingtheprovidedprotocol.Dilutethereaction1:100withSuperOptimalBrothwith20mMglucose(SOC)atroomtemperature.100ulofthedilutionisplatedinduplicateonLBagarplateswith50mg/mlampicillin.Theplatesareincubatedat37°Covernightandtheefficiencyiscalculatedbasedontheaveragenumberofcoloniesperplate.
Format:
Aqueousbuffersolution
Instructionsforuse:
Seedatasheetfordetailedprotocol.
Storage/StABIlity:
Storecompetentcellsat-80°Cuponreceipt.Avoidfreeze/thawcycles.Uponfirstthaw,storeinsingleusealiquots.Stableforatleast6monthsfromdateofreceipt,whenstoredasdirected.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Mol.Biol.CompetentCells/Buffer
ProductType:
Cells
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
