Bpsbioscience/G9a (EHMT2), GST-tag (E. coli-derived)/51000/50 µg
商品编号：
51000
品牌：
bpsbioscience
市场价：
¥7200.00
美元价：
4320.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:74.6kDa
Genbank#:NM_006709
Tag(s):N-terminalGST-tag
a.a:785–1210
Background:
EHMT2(G9a)isalysine-preferringhistonemethyltransferase.EHMT2canmethylateitselfatK165andthisautomethylationisnecessaryandsufficienttomediateinvivointeractionofEHMT2withheterochromatinprotein-1(HP1)(1).EHMT2isresponsIBLeforthemajorityofdimethylationofhistoneH3atlysine9(H3K9me2)andisrequiredfortheefficientrepressionofdevelopmentallyregulatedgenesduringembryonicstemcelldifferentiation(2).EHMT2isoverexpressedinvariouscancersandcancanspecificallymethylatep53atLys(373).EHMT2polymorphismshavebeenassociatedwithcolorectalcancer.
Description:
HumanG9a,alsoknownasEHMT2(euchromatichistone-lysineN-methyltransferase2)andKMT1C(GenBankAccessionNo.NM_006709),a.a.785-1210,withN-terminalGST-tag,MW=74.6kDa,expressedinanE.coliexpressionsystem.
UniProt
Q96KQ7
Synonym(s):
G9a,EHMT2,KMT1C,euchromatichistone-lysineN-methyltransferase2
SpecificActivity:
22pmol/min/µg
AssayConditions:
50µlreactionmix(50mMTrispH8.8,5mMMgCl2,4mMDTT,20µMS-adenosylmethionineand0-40ngG9a)isaddedtowellscoatedwiththesubstrate.Incubatefor1hour.AddantibodyagainstmethylatedK9residueofhistoneH3,incubateatroomtemperaturefor1hour.Finally,incubate1hourwiththeEuropium-labeledsecondaryantibody.Timeresolvedfluorescencemeasuredatroomtemperature(λexc=330nm,λem=620nm).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and16mMglutathione
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Yuan,X.etal.,Mol.Cell27,4,585-595,2007.
2.Nishida,M.etal.,GenesCells12,7,877-888,2007.
ApplicationReference(s):
Onthehistonelysinemethyltransferaseactivityoffungalmetabolitechaetocin(2013)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
