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4000-520-616
当前位置: 首页 > 产品中心 > Other_kits > Bpsbioscience/EZH2-EED Binding Assay Kit/52066/384 reactions
商品详细Bpsbioscience/EZH2-EED Binding Assay Kit/52066/384 reactions
Bpsbioscience/EZH2-EED Binding Assay Kit/52066/384 reactions
Bpsbioscience/EZH2-EED Binding Assay Kit/52066/384 reactions
商品编号: 52066
品牌: bpsbioscience
市场价: ¥29900.00
美元价: 17940.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Background:
EZH2isahistonemethyltransferasethatexhibitsmethylationactivitytowardH3-K27
Description:
TheEZH2-EEDBindingAssayKitisdesignedtomeasuretheinhibitionoftheprotein-proteininteractionbetweenEZH2andEED.ThekeytothiskitisthespecificbindingofEZH2toEED.Withthiskit,onlytwosimplestepsonamicrotiterplatearerequired.First,incubateasamplecontainingEZH2,EEDandaninhibitorofchoice.Next,incubatewithamixtureofacceptoranddonorbeads,followedbyreADIngtheAlpha-counts.
Synonym(s):
KTM6A,EZH2,PRC2,EnhancerOfZesteHomolog2,EZH
SuppliedAs:
ThekitcomesinaconvenientAlphaLISA®format,withGST-taggedEZH2,FLAG-taggedEED,andenoughassaybuffertoperformatotalof384reactions.
AssayConditions:
AssaywasdoneusingAlphaLISAformattomeasuretheinhibitionoftheprotein-proteininteractionbetweenEZH2andEED.Reactionwasperformedatroomtemperaturefor1hour.Anti-FLAGAcceptorBeadsandGlutathioneDonorBeadswereaddedandthereactionwasincubatedfor1houratroomtemperature.AlphaCountswerereadonAlphaScreenmicroplatereader.
Format:

COMPONENTS:

Instructionsforuse:
Seeproductdatasheetfordetailedassayprotocol.
Storage/StABIlity:
Atleastoneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
UsefulforthestudyofEZH2bindingassays,screeninginhibitorsandselectivityprofiling.
Reference(s):
Kong,X.,etal.,J.Med.Chem.2014;57:9512.
Notes:

MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
Anti-FLAGAlphaLISA®AcceptorBeadsBeads,5mg/ml(Perkinelmer#AL112C)
AlphaScreen®GlutathioneDonorBeads,5mg/ml(PerkinElmer#6765300)
Optiplate-384(PerkinElmer#6007290)AlphaScreen®microplatereader
Adjustablemicropipettorandsteriletips

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。