Bpsbioscience/EZH2(Y641F) TR-FRET Assay Kit/52680/384 reactions
商品编号：
52680
品牌：
bpsbioscience
市场价：
¥29900.00
美元价：
17940.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
TheEZH2(Y641F)TR-FRETAssayKitisdesignedtomeasureactivityofthemutantEZH2complex(EZH2(Y641F)/EED/SUZ12/RbAp48/AEBP)inahomogeneous384reactionformat.ThisFRET-basedassayrequiresnotimeconsumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.TheEZH2(Y641F)TR-FRETAssayKitcomesinaconvenientformat,withhistoneH3peptidesubstrate,aTb-labeledantibodyagainstmethylatedK27residueofHistoneH3,S-adenosylmethionine,methyltransferaseassaybuffer,TR-FRETdetectionbuffer,dye-labeledacceptor,andpurifiedEZH2(Y641F)complexfor384enzymereactions.ThekeytotheEZH2(Y641F)AssayKitisahighlyspecificantibodythatrecognizesmethylatedHistoneH3K27.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,S-adenosylmethionineisincubatedwithasamplecontainingassaybufferandmethyltransferaseenzymefor240minutes.Next,antibodyisadded.Finally,dye-labeledacceptorisaddedfollowedbyfluorescencereADIng.
UniProt
Q15910
Synonym(s):
EZH2(Y641F),EED,SUZ12,RbAp48,AEBP
SuppliedAs:
TheEZH2(Y641F)TR-FRETAssayKitcomesinaconvenientformatfor384enzymereactions.
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
1.Dillon,S.C.,etal.GenomeBIOLOGy2005;6:227.
2.Morin,R.D.,etal.NatGenet.2010,42(2):181.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
