Bpsbioscience/EZH2 / EED / SUZ12, His-tag, FLAG-tag/51003/50 µg
商品编号：
51003
品牌：
bpsbioscience
市场价：
¥12600.00
美元价：
7560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 224 kDa (complex)
Genbank #: NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12)
Tag(s): N-terminal His-tag (EZH2, SUZ12), N-terminal FLAG-tag (EED)
a.a: 2–end (all components)
Background:
EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Description:
Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-tag, MW= 86 kDa, human EED (GenBank Accession No. NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, and human SUZ12 (GenBank Accession No. NM_015355), a.a. 2-end with N-terminal His-tag, MW = 87 kDa, co-expressed in a baculovirus expression system.
UniProt
Q15910
Synonym(s):
KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2, EZH
Specific Activity:
0.08 pmole/min/µg
Assay Conditions:
50 µl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 µM S-adenosylmethionine, and 250-2000 ng enzyme complex) is added to the wells coated with histone H3 substrate on a Neutravidin black plate (ThermoFisher). Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Add HRP chemiluminescent substrates and read luminescence.
Format:
Aqueous buffer solution
Storage / Stability:
≥ 6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008).
2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).
Application Reference:
Pint lincRNA connects the p53 pathway with epigenetic silencing by the Polycomb repressive complex 2
(2013)
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
