Bpsbioscience/EZH2 (Y641N) /EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag/51028/20 µg
商品编号：
51028
品牌：
bpsbioscience
市场价：
¥7700.00
美元价：
4620.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:325kDa(complex)
Genbank#:NM_004456(EZH2),NM_003797(EED),NM_015355(SUZ12),NM_153207(AEBP2),NM_005610(RbAP48)
Tag(s):N-terminalHis-tag(EZH2,SUZ12,RbAp48,AEBP2),N-terminalFLAG-tag(EED)
a.a:2–end(allcomponents)
Background:
EZH2isthecatalyticsubunitofthePRC2/EED-EZH2complex,whichmethylatesLys9(H3K9me)andLys27(H3K27me)ofhistoneH3,leADIngtotranscriptionalrepressionoftheaffectedtargetgene.Abletomono-,di-andtrimethylateLys27ofhistoneH3toformH3K27me1,H3K27me2andH3K27me3,respectively.ComparedtoEZH2-containingcomplexes,itismoreabundantinembryonicstemcellsandplaysamajorroleinformingH3K27me3,whichisrequiredforembryonicstemcellidentityandproperdifferentiation.ThePRC2/EED-EZH2complexmayalsoserveasarecruitingplatformforDNAmethyltransferases,therebylinkingtwoepigeneticrepressionsystems.GenesrepressedbythePRC2/EED-EZH2complexincludeHOXC8,HOXA9,MYT1,CDKN2Aandretinoicacidtargetgenes.EZH2canalsomethylatenon-histoneproteinssuchasthetranscriptionfactorGATA4.
Description:
MutantversionofourregularEZH25-membercomplex(Cat.#51004),butwithaTyr-to-Asnmutationona.a.646oftheEZH2protein.ComplexofhumanEZH2(GenBankAccessionNo.NM_004456),a.a.2-end,Y646N*,withN-terminalHis-tag,MW=86kDa,humanEED(NM_003797),a.a.2-endwithN-terminalFLAG-tag,MW=51kDa,humanSUZ12(NM_015355),a.a.2-endwithN-terminalHis-tag,MW=87kDa,HumanAEBP2(NM_153207),a.a.2-endwithN-terminalHis-tag,MW=53kDa,andhumanRbAp48(NM_005610),a.a.2-endwithN-terminalHis-tag,MW=48kDa,co-expressedinabaculovirusexpressionsystem.
UniProt
Q15910
Synonym(s):
KTM6A,EZH2,PRC2,EnhancerOfZesteHomolog2,EZH2(Y646N),EZH,RetinoblastomaBindingProtein4(RBBP4)
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80ng/?lFLAGpeptide,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
≥ 6monthsat-80°C
Application(s):
UsefulforWesternblotanalysisandtoinvestigateprotein-proteininteractionsintheEZH2complex.
Reference(s):
1.Varambally,S.etal.Science322 (5908),1695-1699(2008).
2.Rakotobe,D.etal.,Virol.J.5,32 (2008).
ApplicationReference:
Morin,R.D., etal.NatGenet. 2010February; 42(2):181–185.
Notes:
ThismutationisoftencitedinthescientificliteratureasY641Ninvariant3GenBankAccessionNo.NM_001203247).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
