Bpsbioscience/EZH1/EED/SUZ12/RbAp48/AEBP2，His标签/51007/50µg
商品编号：
51007
品牌：
bpsbioscience
市场价：
¥19600.00
美元价：
11760.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:325kDa(complex)
Genbank#:NM_001991(EZH1),NM_003797(EED),NM_015355(SUZ12),NM_153207(AEBP2),NM_005610(RbAP48)
Tag(s):N-terminalHis-tag
a.a:2–end(allcomponents)
Description:
ComplexofhumanEZH1,GenBankAccessionNo.NM_001991,a.a.2-end,withN-terminalHis-tag,MW=86kDa,humanEED,NM_003797,a.a.2-end,withN-terminalFLAG-tag,MW=51kDa,humanSUZ12,NM_015355,a.a.2-end,withN-terminalHis-tag,MW=87kDa,humanAEBP2,NM_153207,a.a.2-end,withN-terminalHis-tag,MW=53kDa,andhumanRbAp48,NM_005610,a.a.2-end,withN-terminalHis-tag,MW=48kDa,co-expressedinbaculovirusexpressionsystem.
UniProt
Q92800
Synonym(s):
KMT6B,EZH,RetinoblastomaBindingProtein4(RBBP4)
AssayConditions:
50µlreactionmix(20mMphosphatepH7.4,0.05%Tween-20,40µMS-adenosylmethionine,andenzymecomplex)addtothewellscoatedwiththesubstrateonaNeutravidinwhiteplate(G-Biosciences).Incubatefor1hr.AddantibodyagainstmethylatedK27residueofhistoneH3,incubate1hr.Then,addsecondaryHRP-labeledantibodyandincubate30min.Finally,addHRPchemiluminescentsubstratesandreadluminescence.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,200mMimidazole,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.MargueronR.etal.Mol.Cell32,4,503-518,2008.
2.ShenX.etal.,Mol.Cell32,4,491-502,2008.
2.ShenX.etal.,Mol.Cell32,4,491-502,2008.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
