Bpsbioscience/EHMT1 (GLP), GST-tag/51020/50 µg
商品编号：
51020
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 72.4 kDa
Genbank #: NM_024757
Tag(s): N-terminal GST-tag
a.a: 894–1298 (end)
Description:
Human euchromatic histone-lysine N-methyltransferase (EHMT1), also known as G9a-like protein, GLP or KMT1D, GenBank Accession No. NM_024757, a.a. 894-1298 (end) with N-terminal GST-tag, MW= 72.4 kDa, expressed in an E. coli expression system.
UniProt
Q9H9B1
Synonym(s):
EHMT1, GLP, KMT1D, G9a-like protein, euchromatic histone-lysine N-methyltransferase
Specific Activity:
≥122.3 pmole/min/µg
Assay Conditions:
50 μl reaction mix (50 mM Tris pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 40 μM S-adenosylmethionine, and EHMT1 (0.05 – 0.5 ng)) is added to substrate-coated wells on a Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41.
2. Ueda, J., et al., J Biol. Chem. 2006 Jul 21; 281(29): 20120-8.
2. Ueda, J., et al., J Biol. Chem. 2006 Jul 21; 281(29): 20120-8.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Methyltransferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
