Bpsbioscience/SetDB1 Chemiluminescent Assay Kit/51056/96 reactions
商品编号：
51056
品牌：
bpsbioscience
市场价：
¥13900.00
美元价：
8340.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
TheSetDB1ChemiluminescentAssayKitisdesignedtomeasureSetDB1activityforscreeningandprofilingapplications.ThekeytotheSetDB1DirectActivityAssayKitisahighlyspecificantibodythatrecognizesthemethylatedK9residueofHistoneH3.Withthiskit,onlythreesimplestepsarerequiredformethyltransferasedetection.First,S-adenosylmethionineisincubatedwithasamplecontainingassaybufferandmethyltransferaseenzyme.Next,primaryantibodyisadded.Finally,thestripsaretreatedwithanHRP-labeledsecondaryantibodyfollowedbyadditionoftheHRPsubstratetoproducechemiluminescencethatcanthenbemeasuredusingachemiluminescencereader.
UniProt
Q15047
Synonym(s):
Histone-lysineNmethyltransferaseSETDB1,SETdomainbifurcated1,KMT1E,ERG-associatedproteinwithSETdomain(ESET)
SuppliedAs:
Kitcomesinaconvenientformat,withwellsprecoatedwithhistoneH3peptidesubstrate,antibodyagainstmethylatedlysineresidueofHistoneH3,secondaryHRP-labeledantibody,S-adenosylmethionine,methyltransferasebuffer,andpurifiedenzyme
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Oneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
DillonSC,ZhangX,TrievelRC,ChengX.GenomeBIOLOGy2005;6:227.
Notes:
MATERIALSREQUIREDBUTNOTSUPPLIED:
TBSTbuffer(1xTBS,pH8.0,containing0.05%Tween-20)
LuminometerorfluorescentmicroplatereadercapableofreADIngchemiluminescence
Adjustablemicropipettorandsteriletips
Rotatingorrockerplatform
TBSTbuffer(1xTBS,pH8.0,containing0.05%Tween-20)
LuminometerorfluorescentmicroplatereadercapableofreADIngchemiluminescence
Adjustablemicropipettorandsteriletips
Rotatingorrockerplatform
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferases
ProductType:
AssayKits
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
