Bpsbioscience/PKLR Var2 (PKL), His-tag/40501/20 µg
商品编号：
40501
品牌：
bpsbioscience
市场价：
¥9400.00
美元价：
5640.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:59kDa
Genbank#:NM_181871
Tag(s):N-terminalHis-tag
a.a:2–543(end)
Background:
PKLRisapyruvatekinasewhichcatalyzesthetransphosphorylationofphohsphoenolpyruvateintopyruvateandATP.Thisreactionistherate-limitingstepofglycolysis.PKLRgeneencodestheL-andR-typeisoenzymesthroughalternatesplicingeventscontrolledbydifferentpromoters.L-typeisoformcanalsoappearasatetramerandisupregulatedbyglucosewithimplicationsinmaturity-onsetdiabetesoftheyoung.
Description:
Humanpyruvatekinasevariant2(PKL),GenBankAccessionNo.NM_181871,fulllengthwithN-terminalHis-tag2-543(end),MW=59kDa,expressedinanE.coliexpressionsystem.
UniProt
P30613
Synonym(s):
PyruvateKinaseRBC,PKLRVar2,PKL,PK1
SpecificActivity:
≥190,000pmol/min/µg.
AssayConditions:
A50µlreactionisconductedinabuffercontaining50mMTris(pH7.4),10mMMgCl2,50mMKCl,2mMADP,10mMphosphoenolpyruvate(PEP)atroomtemperaturefor15min.ATPproductionisdetectedusingKinase-Glo®LuminescentKinaseAssayPlatform.
Formulation:
40mMTrispH8.0,146mMNaCl,20%glycerol,and3mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.BullidoM.J.etal.,Neurobiol.Aging.29(10):1160-6(2008).
2.RussellS.T.etal.,Exp.Cell.Res.315(1):16-25(2009).
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Kinase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
