Bpsbioscience/NAMPT (PBEF1), GST-tag/91004/50 µg
商品编号：
91004
品牌：
bpsbioscience
市场价：
¥8000.00
美元价：
4800.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:82kDa
Genbank#:NM_005746
Tag(s):N-terminalGST-tag
a.a:2–491(end)
Description:
HumanNicotinamidephosphoribosyltransferaseprotein(NAMPT),alsoknownasPBEF1orvisfatin(GenBankAccessionNo.NM_005746)a.a.2-491,withN-terminalGST-tag,MW=82kDa,expressedinanE.colicellexpressionsystem.
UniProt
P43490
Synonym(s):
Nicotinamidephosphoribosyltransferaseprotein,NAMPT,PBEF1,visfatin,pre-B-cellcolony-enhancingfactor1,NMPT
SpecificActivity:
≥0.6pmol/min/µg
AssayConditions:
Thereactionmixturecontained50mMTris-HCl,pH8.0,12.5mMMgCl2,0.1mMnicotinamide,0.4mMPRPP,2mMATP,30µg/mlofalcoholdehydrogenase,10µg/mlofNMNAT(BPS,#71090),1.5%alcohol,1mMDTT,0.02%BSA,andvariousamountofNAMPTinafinalvolumeof50µl.ReactionswereinitiatedwiththeadditionofNAMPT,andtheincreaseinNADHfluorescence(Ex340nm/Em460nm)wascontinuouslymonitoredfor30minatroomtemperature.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,10mMglutathione,0.04%Tween-20,20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofproteinbindingassays,enzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.AmalBetal.,Mol.Cell.Biol.14(2)
2.MartinPR.etal.,J.Bacteriol.183(4)
2.MartinPR.etal.,J.Bacteriol.183(4)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
MetabolicEnzymes-Transferase
ProductType:
RecombinantProtein
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品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
