Bpsbioscience/NAMPT(PBEF1)/71098/50µg
商品编号:
71098
品牌:
bpsbioscience
市场价:
¥8000.00
美元价:
4800.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 56 kDa
Genbank #: NM_005746
Tag(s): none
a.a: 2–491(end)
Background:
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging.
Description:
Human Nicotinamide phosphoribosyltransferase protein (NAMPT), also known as PBEF1 or visfatin (GenBank Accession No. NM_005746), a.a. 2-491 (end), no tag, MW=56 kDa, expressed in an E. coli cell expression system.
UniProt
P43490
Synonym(s):
Nicotinamide phosphoribosyltransferase protein, NAMPT, PBEF1, visfatin, Pre-B Cell-Enhancing Factor
Specific Activity:
≥ 2 pmol/min/µg
Assay Conditions:
The 50 µl reaction mixture contained 50 mM Tris-HCl, pH 8.0, 12.5 mM MgCl2, 0.1 mM nicotinamide, 0.4 mM PRPP, 2 mM ATP, 30 µg/ml of alcohol dehydrogenase, 10 µg/ml of NMNAT (BPS, #71090), 1.5% alcohol, 1 mM DTT, 0.02% BSA. Reactions were incubated at room temperature for 30 minutes, and the increase in NADH fluorescence (Ex 340 nm/Em 460 nm) was measured.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Metabolic Enzymes-Transferase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。