Bpsbioscience/IDH2, FLAG-tag/71074-2/100 µg
商品编号：
71074-2
品牌：
bpsbioscience
市场价：
¥9600.00
美元价：
5760.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 52 kDa
Genbank #: NM_002168
Tag(s): C-terminal FLAG-tag
a.a: 1–452(end)
Description:
Human isocitrate dehydrogenase 2, also known as IDH2, (GenBank Accession No. NM_002168), a.a. 1-452(end) with C-terminal FLAG-tag, MW = 52 kDa, expressed in a baculovirus infected Sf9 cell expression system.
UniProt
P48735
Synonym(s):
Isocitrate Dehydrogenase 2, IDH2, oxalosuccinate decarboxylase
Specific Activity:
≥14,200 pmol/min/µg.
Assay Conditions:
The reaction mixture contained 50 mM Tris, pH 7.4, 5 mM MgCl2, 200 µM DL-isocitrate, 200 µM NADP+, and various amount of IDH2 in a volume of 200 µL. Reactions were initiated with the addition of IDH2, and the increase in NADPH absorbance at 340 nm was continuously monitored for 7 min. Molar extinction coefficient of NADPH is 6,220 M-1cm-1.
Formulation:
45 mM Tris-HCl, pH 8.0,
124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAG
peptide, 3 mM DTT and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at –80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Schaap, F.G., et al., Adv Anat Pathol 2013;20(1):32-38.
2. Grassian, A.R., et al., J Biol Chem 2012;287(50):42180-42194.
2. Grassian, A.R., et al., J Biol Chem 2012;287(50):42180-42194.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Metabolic Enzymes-Dehydrogenase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
