Bpsbioscience/IDH2（R140Q），标志标签/71100-1/50µg
商品编号：
71100-1
品牌：
bpsbioscience
市场价：
¥5500.00
美元价：
3300.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:52kDa
Genbank#:NM_002168
Tag(s):C-terminalFLAG-tag
a.a:1–452(end)
Background:
Isocitratedehydrogenasescatalyzetheoxidativedecarboxylationofisocitrateto2-oxoglutarate.Theseenzymesbelongtotwodistinctsubclasses,oneofwhichutilizesNAD(+)astheelectronacceptorandtheotherNADP(+).TheIDH2homodimerisantheNADP(+)-dependentisocitratedehydrogenasefoundinthemitochondria.Itplaysaroleinintermediarymetabolismandenergyproductionandmaytightlyassociateorinteractwiththepyruvatedehydrogenasecomplex.
Description:
Humanisocitratedehydrogenase2,alsoknownasIDH2,GenBankAccessionNo.NM_002168,a.a.1-452(end)withwithArgtoGlnmutationona.a.140andC-terminalFLAG-tag,MW=52kDa,expressedinabaculovirusinfectedSf9cellexpressionsystem.
UniProt
P48735
Synonym(s):
IsocitrateDehydrogenase2,IDH2,oxalosuccinatedecarboxylase,IDHM
SpecificActivity:
≥61pmol/min/µg
AssayConditions:
IDHreductiveactivitywasmeasuredin200µlreactioncontaining25mMTris(pH7.4),150mMNaCl,10mMMgCl2,0.03%BSA,1mMalpha-Ketoglutarate,10µMNADPHandIDH.DepletionofNADPHwasmonitoredcontinuouslyatAbs340nmfor20min.MolarextinctioncoefficientofNADPHis6,200M-1cm-1.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80μg/mlFLAGpeptide,0.04%Tween-20,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Schaap,F.G.,etal.,Adv.Anat.Pathol.2013;20(1):32-38.
2.Grassian,A.R.,etal.,J.Biol.Chem.2012;287(50):42180-42194.
2.Grassian,A.R.,etal.,J.Biol.Chem.2012;287(50):42180-42194.
Notes:
ThisproteinrunsaberrantlybySDS-PAGE.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
MetabolicEnzymes-Transferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
