Bpsbioscience/IDH1 (R132H), FLAG-tag/71099-1/50 µg
商品编号：
71099-1
品牌：
bpsbioscience
市场价：
¥5500.00
美元价：
3300.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:48kDa
Genbank#:NM_005896
Tag(s):C-terminalFLAG-tag
a.a:1–414(end)
Background:
Isocitratedehydrogenasescatalyzetheoxidativedecarboxylationofisocitrateto2-oxoglutarate.Theseenzymesbelongtotwodistinctsubclasses,oneofwhichutilizesNAD(+)astheelectronacceptorandtheotherNADP(+).IDH1homodimerisanNADP(+)-dependentisocitratedehydrogenasefoundinthecytoplasmandperoxisomes.ItcontainsthePTS-1peroxisomaltargetingsignalsequence.ThepresenceofthisenzymeinperoxisomessuggestsrolesintheregenerationofNADPHforintraperoxisomalreductions,aswellasinperoxisomalreactionsthatconsume2-oxoglutarate.ThecytoplasmicenzymeservesasignificantroleincytoplasmicNADPHproduction.
Description:
HumanIsocitrateDehydrogenase(IDH1),alsoknownasoxalosuccinatedecarboxylase,GenBankAccessionNo.NM_005896,fulllength[a.a.1-414(end)]withArgtoHismutationona.a.132andC-terminalFLAG-tag,MW=48kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
O75874
Synonym(s):
IsocitrateDehydrogenase1,IDH1,oxalosuccinatedecarboxylase,IDP
SpecificActivity:
≥53pmol/min/µg
AssayConditions:
IDHreductiveactivitywasmeasuredin200µlreactioncontaining25mMTris(pH7.4),150mMNaCl,10mMMgCl2,0.03%BSA,1mMalpha-Ketoglutarate,10?MNADPHandIDH.DepletionofNADPHwasmonitoredcontinuouslyatAbs340nmfor20min.MolarextinctioncoefficientofNADPHis6,200M-1cm-1.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80µg/mlFLAGpeptide,0.04%Tween-20and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Dang,L.,etal.(2010).TrendsMol.Med.16(9):387-97.
2.Paschka,P.,etal.(2010).J.Clin.Oncol.28(22):3636-43.
2.Paschka,P.,etal.(2010).J.Clin.Oncol.28(22):3636-43.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
MetabolicEnzymes-Transferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
