Bpsbioscience/GLS1，His标签/71102/20µg
商品编号：
71102
品牌：
bpsbioscience
市场价：
¥9600.00
美元价：
5760.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:72.5kDa
Genbank#:NM_014905
Tag(s):N-terminalHis-tag
a.a:17–669(end)
Background:
GLS1isaphosphate-activatedamidohydrolasethatcatalyzesthehydrolysisofglutaminetoglutamateandammonia.GLS1isprimarilyexpressedinthebrainandkidneywhereitcatalyzesthefirstreactionintheprimarypathwayfortherenalcatabolismofglutamine.Itplaysanessentialroleingeneratingenergyformetabolism,synthesizingthebrainneurotransmitterglutamateandmaintainingacid-basebalanceinthekidney.Alternatesplicingresultsinmultipletranscriptvariants.
Description:
Humanglutaminase(GLS),alsoknownasglutaminasetranscriptvariant1(GLS1),GAC,GAMandKGA,GenBankAccessionNo.NM_014905,a.a.17-669(end),withN-terminalHis-tag,MW72.5kDa,expressedinanE.colicellexpressionsystem.
UniProt
O94925
Synonym(s):
glutaminase,glutaminasetranscriptvariant1,GLS1,GAC,GAM,KGA,GLS
SpecificActivity:
≥25nmol/min/µg
AssayConditions:
Thereactionmixturecontained100mMTris,pH8.6,150mMK2HPO4,25mML-glutamine,2mMNAD+,4unitsofGDH,andvariousamountsofGLS1inavolumeof200?l.ReactionswereinitiatedwiththeadditionofGLS1,andtheincreaseinNADHabsorbanceat340nmwascontinuouslymonitoredfor7minutesat37°C.MolarextinctioncoefficientofNADHis6,220M-1cm-1.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.VandenHeuvel,A.P.,etal.,CancerBiol.Ther.2012;13(12):1185-1194.
2.Thangavelu,K.,etal.,Proc.Natl.Acad.Sci.USA2012;109(2):7705-7710.
2.Thangavelu,K.,etal.,Proc.Natl.Acad.Sci.USA2012;109(2):7705-7710.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
MetabolicEnzymes-Transferase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
