Bpsbioscience/cKit（V654A），His标签/40251/10µg
商品编号：
40251
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:53.4kDa
Genbank#:NM_000222
Tag(s):N-terminalHis-tag
a.a:544–976(end)
Description:
HumanKit(V654A)(GenBankAccessionNo.NM_000222),a.a.544-976(end)withsubstitutionofvaline654toalanineandN-terminalHis-tag,MW=53.4kDa,expressedinanBaculovirusinfectedSf9cellexpressionsystem.
UniProt
P10721
Synonym(s):
KIT,cKIT,c-KIT,PBT,CD117,PiebaldTrait,SCFR,Mast/StemCellGrowthFactorReceptor
SpecificActivity:
89pmol/min/μg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillphosphorylate1nmoloftyrosinesubstrateperminuteatpH7.4and30°C.
AssayConditions:
Enzymereactionisconductedinabuffercontaining50mMHEPES,pH7.4,3mMMgCl2,3mMMnCl2,1mMDTT,3µMNaorthovanadate,0.1mMATP,30µg/mlPoly(Glu:Tyr)4:1substrate,and4µg/mlrecombinantcKit.Assaywasdonewith0.2mg/mlPoly-(Glu-Tyr)peptideusingADPGlo®(Promega)conditionsat30°Cfor35minutes.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Schumaker,J.A.,etal.(2008)J.Clin.Pathol.61:109-114.
2.Sun,J.etal.(2009)J.Biol.Chem.284:11039-11047.
2.Sun,J.etal.(2009)J.Biol.Chem.284:11039-11047.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/ReceptorTyrosine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
