Bpsbioscience/CDK9/CyclinT1, GST-tag/40307/10 µg
商品编号：
40307
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 cell
MW: CDK9: 69 kDa; CyclinT1: 107 kDa
Genbank #: CDK9: NM_001261; CyclinT1: NM_001240
Tag(s): N-terminal GST-tag
a.a: CDK9: 2- 372(end); CyclinT1: 2-726(end)
Description:
Human cyclin-dependent kinase 9 (CDK9), also known as C-2K and cell division protein kinase 9, complexed with human cyclin T1 (CCNT1, CycT1), GenBank Accession Nos. NM_001261 (CDK9) and NM_001240 (CyclinT1), a.a. 2- 372(end) (CDK9) and a.a. 2-726(end) (CyclinT1) each with N-terminal GST-tags, co-expressed in a baculovirus-infected Sf9 cell expression system. MW = 69 kDa (CDK9) and 107 kDa (CyclinT1).
UniProt
CDK9: P50750; CyclinT1: O60563
Synonym(s):
cyclin-dependent kinase 9, C-2K, cell division protein kinase 9, and CCNT1 , CycT1
Specific Activity:
26 pmol/min/μg
Assay Conditions: Assay was done in a
Kinase buffer using PDKtide (0.1 mg/ml) as
a substrate with 10 M ATP at 30°C for 45
min. The amount of ATP transferred from
autophosphorylation as well as substrate
phosphorylation was calculated using
Kinase-Glo reagent (Promega).
Formulation:
40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 0.04%
Tween-20, 3 mM DTT, 20% glycerol, and
1.6 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Yang, Z., et al., Nat. 2001; 414: 317-322.
2. Baumli, S., et al., EMBO J. 2008; 27(13): 1907-1918.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted
enzyme is not recommended, if
necessary, use carrier protein (BSA 0.1
– 0.5%).
Scientific Category:
Kinase - Serine/Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
