Bpsbioscience/CDK5/p35，GST标签/40095/10µg
商品编号：
40095
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:108kDa(complex)
Genbank#:NM_004935(CDK5),NM_003885(p35)
Tag(s):N-terminalGST-tag
a.a:fulllength
Background:
CDK5isamemberoftheCyclin-DependentKinasefamilythatismostabundantinthemammalianbrain.ActiveformofCDK5,whichhasalsobeencalledneuronalcdc2-likekinase,isaheterodimerofCDK5anda25kDaproteinwhichisderivedproteolyticallyfroma35kDabrainandneuron-specificproteinandisessentialforthekinaseactivityofCDK5(1).CDK5hasemergedasacrucialregulatorofneuronalmigrationinthedevelopingcentralnervoussystem.CDK5phosphorylatesadiverselistofsubstrates,implicatingitintheregulationofarangeofcellularprocesses-fromadhesionandmotility,tosynapticplasticityanddrugaddiction(2).
Description:
Recombinantfull-lengthhumanCDK5(59kDa)andhumanp35(60kDa),bothwithN-terminalGST-tags,wereco-expressedinSf9cellsusingabaculovirusexpressionsystem.TheCDK5geneaccessionnumberisNM_004935andp35geneaccessionnumberisNM_003885.
UniProt
Q00535(CDK5),Q15078(p35)
Synonym(s):
Cyclin-DependentKinase5,CellDevisionKinase5,CDK5,p35,Cyclin-DependentKinase5RegulatorySubunit1,CDK5R1
SpecificActivity:
180pmol/min/µg
AssayConditions:
AssaywasperformedinKinasebuffercontaining0.2mMDTTusingHistone1substrate(0.2mg/ml)and20mMATP.Reactionwasperformedat30°Cfor45min.AmountofATPtransferredwascalculatedusingKinase-Glo®reagent(Promega).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glyceroland3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Tang,D.etal.ProgCellCycleRes.1996,2:205-16.
2.Dhavan,R.etal.NatRevMolCellBiol.2001Oct,2(10):749-59.
2.Dhavan,R.etal.NatRevMolCellBiol.2001Oct,2(10):749-59.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
