Bpsbioscience/BRAF, GST-tag/40065/10 µg
商品编号：
40065
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 66 kDa
Genbank #: NM_004333
Tag(s): N-terminal GST-tag
a.a: 417–766 (end)
Description:
Human BRAF (b-RAF) 9GenBank Accession #NM_004333), a.a. 417-766 (end) with N-terminal GST-tag, MW = 66 kDa, expressed in Sf9 cells using a Baculovirus expression system.
UniProt
P15056
Synonym(s):
B-RAF1, BRAF, RAFB1
Specific Activity:
44 pmol/min/µg
Assay Conditions:
Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA)containing 2 μg inactive MEK1 (BPS #40075), 5 μM ATP, and 0.1 – 1.6 ng/μl BRAF for 60 minutes at room temperature. The amount of ATP transferred was measured by BPS ATP quantification reagents.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775–1780.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.
2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
