Bpsbioscience/BRAF (V600E), GST-tag/40533/10 µg
商品编号：
40533
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:69kDa
Genbank#:NM_004333
Tag(s):N-terminalGST-tag
a.a:417–end
Background:
BRAFisamemberoftheRAFfamilythatisactivatedbymembersoftheRasfamilyupongrowthfactor-inducedstimulation.ActiveRascaninduceheterodimerizationofcRafandBRAFandthismayexplaintheobservedcooperativityofcRafandBRafincellsrespondingtogrowthfactorsignals(1).ActivatingmutationsintheBRAFgenearepresentinalargepercentageofhumanmalignantmelanomasandinaproportionofcoloncancers.Thevastmajorityofthesemutationsresultinavalinetoglutamicacidchangeatresidue599withintheactivationsegmentofB-RAF(2).
Description:
Humanv-rafmurinesarcomaviraloncogenehomologB1(BRAF),GenBankAccessionNo.NM_004333,a.a.417-endwithVal-to-Glumutationona.a.600*andN-terminalGST-tag,MW=69kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.*ThismutationisoftencitedasV600E.
UniProt
P15056
Synonym(s):
BRAF1,RAFB1,B-raf1,MGC126806,MGC138284,BRAF(V600E)
SpecificActivity:
150pmol/min/μg
AssayConditions:
Assaywasperformedinakinasebuffercontaining0.1mg/mlinactiveMEK1substrate(BPSCat#40075)and10μMATP.Reactionwasdoneat30°Cfor30minutes.ATPtransferredwascalculatedusingKinase-Glo®(Promega).
Formulation:
40mMTris,pH8.0,110mMNaCl,2.2mMKCl,3mMDTTand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Weber,C.K.,etal.,CancerRes.2001May1,61(9):3595-8.
2.Mercer,K.E.,etal.,BiochimBiophysActa.2003Jun5,653(1):25-40.
2.Mercer,K.E.,etal.,BiochimBiophysActa.2003Jun5,653(1):25-40.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
