Bpsbioscience/BMX, GST-tag/40402/10 µg
商品编号：
40402
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 110 kDa
Genbank #: NM_001721
Tag(s): N-terminal GST-tag
a.a: full length
Background:
The BMX gene encodes a non-receptor tyrosine kinase, which may play a role in the growth and differentiation of hematopoietic cells (1). The BMX gene is located on chromosomal band Xp22.2 between the DXS197 and DXS207 loci. Interestingly, chromosome X also contains the closest relative of BMX, the BTK gene, implicated in X-linked agammaglobulinemia. BMX, is found to induce activation of the Stat signaling pathway (2).
Description:
Recombinant full-length
human cytoplasmic tyrosine-protein
kinase BMX (BMX), also known as bone
marrow tyrosine kinase gene in
chromosome X protein, epithelial and
endothelial tyrosine kinase, ETK, and
NTK38 was expressed by baculovirus in
Sf9 insect cells using an N-terminal GST
tag, a.a. 1-675 (end). The gene
accession number is NM_001721
(variant 2).
UniProt
P51813
Synonym(s):
ETK, PSCTK2, PSCTK3, bone marrow tyrosine kinase gene in chromosome X protein, epithelial and endothelial tyrosine kinase, NTK38
Specific Activity:
43 pmol/min/µg
Assay Conditions:
Assay was done in
Kinase buffer containing 0.2 mM DTT
using Poly-(Tyr,Glu) substrate (0.2
mg/ml) and 20 mM ATP. Reaction was
done at 30°C for 45 min. Amount of ATP
transferred was measured using Kinase-
Glo reagent (Promega)
Formulation:
50 mM Tris-HCl, pH 7.5,
150 mM NaCl, 0.25 mM DTT, 0.1 mM
EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25%
glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Tamagnone, L. et al. Oncogene. 1994 Dec;9(12):3683-8.
2. Saharinen, P. et al. Blood. 1997 Dec 1;90(11):4341-53
2. Saharinen, P. et al. Blood. 1997 Dec 1;90(11):4341-53
Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme
is not recommended, if necessary, use
carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Kinase/Cytoplasmic Tyr
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
