Bpsbioscience/CD137/NF-κB报告基因-HEK293重组细胞系/79289/2瓶
商品编号:
79289
品牌:
bpsbioscience
市场价:
¥176000.00
美元价:
105600.00
产品分类:
常规细胞株
公司分类:
Routine_cell_strain
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Background:
HumanCD137(4-1BB;TNFRS9)isaninducIBLeco-stimulatorymoleculethatactivatesTcells.CD137:CD137L-mediatedsignalinghasbeenshowntobeimportantforproliferation,effectorfunctionsandsurvivalsofTcells.CD137isalsoexpressedinNKandNKTcells.AccumulatingevidenceshowsaroleforCD137:CD137Lsignalingininflammation,suggestingthatinhibitionofthispathwaymayprovideatherapeuticavenuetotreatautoimmuneandinflammatorydiseases.Similarly,antibodiestargetingCD137activationinimmunecellshavedemonstratedpotentanti-tumorpropertiesincancerpatients.
Description:
RecombinantHEK293celllineexpressingafulllengthhumanCD137(NM_003811).TheNF-ĸBluciferasereporterconstructisstablyintegratedintothegenome.Thefireflyluciferasegeneiscontrolledby4copiesofNF-κBresponseelementlocatedupstreamoftheTATApromoter.FollowingactivationbyhumanCD137ligand,NF-ĸBtranscriptionfactorsbindtotheDNAresponseelementstoinducetranscriptionoftheluciferasegene.
MaterialsRequiredButNotProvided:
CD137Lprotein(BPSBioscience,#71189)
Anti-CD137AgoNISTAntibody(BPSBioscience,#79097-2)
ONE-StepLuciferaseDetectionReagent(BPSBioscience,#60690)
ThawMedium1(BPSBioscience,#60187)
Synonym(s):
CD137,4-1BB
HostCellline:
HEK293
GrowthMedia:
ThawMedium1(BPSBioscience,#60187):Thismedium,optimizedforplatingandthawingHEK293cells,includesincludes10%FBS,non-essentialaminoacids,sodiumpyruvate,and1%Penicillin/Streptomycin.
CompleteGrowthMedium:ThawMedium1(BPSBioscience,#60187)plus400µg/mlG418(Geneticin)and50µg/mlHygromycinB(ThermoFisher,#10687010).
Format:
Eachvialcontains2x10^6cellsin1mlof10%DMSOinFBS.
Storage/StABIlity:
Storeinliquidnitrogenimmediatelyuponreceipt.
Application(s):
•ScreenforactivatorsorinhibitorsofCD137signalinginacellularcontext
•CharacterizetheBIOLOGicalactivityofCD137anditsinteractionswithligands
Notes:
Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.
ScientificCategory:
Immunotherapy
ProductType:
CellLine
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。