Bpsbioscience/UTX（KDM6A），N端标志标签/50119/20&mu；G
商品编号：
50119
品牌：
bpsbioscience
市场价：
¥9500.00
美元价：
5700.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Baculovirus-infected Sf9 cell
MW: 155 kDa
Genbank #: NM_021140
Tag(s): N-terminal FLAG-tag
a.a: 1-1401
Description:
Human UTX, also known as KDM6A (GenBank Accession No. NM_021140) (amino-acids 1-1401) with N-terminal FLAG-tag, MW = 155 kDa, expressed in Sf9 cells via a baculovirus expression system.
UniProt
O15550
Synonym(s):
UTX, KDM6A
Specific Activity:
0.096 pmol/min/μg
Assay Conditions:
10 µl reaction mix containing assay buffer with 50 mM HEPES (pH 7.4), 1mM TCEP, 100 µM alpha-ketoglutarate, 25 µM iron, 0.1mM ascorbic acid, 0.02% Tween-20, 0.5 µM biotinylated peptide substrate, and UTX (10 – 50ng) added to the wells. Add antibody against di-methylated K27 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 80 μg/ml flag peptide and 0.2mM TCEP
Storage / Stability:
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Greenfield A, et al. (1998). Hum. Mol. Genet. 7 (4): 737–42.
2. Agger K, et al. (2007). Nature 449 (7163): 731–4.
Application Reference(s):
1. Inhibition of KDM4A activity as a strategy to suppress interleukin-6 production and attenuate colitis induction (2017)
2. Identification of the KDM2/7 histone lysine demethylase subfamily inhibitor and its antiproliferative activity (2013)
Warning(s):
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
