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当前位置: 首页 > 产品中心 > Other_kits > Bpsbioscience/LSD2(KDM1B)均相分析试剂盒(一甲基)/50613/384反应
商品详细Bpsbioscience/LSD2(KDM1B)均相分析试剂盒(一甲基)/50613/384反应
Bpsbioscience/LSD2(KDM1B)均相分析试剂盒(一甲基)/50613/384反应
Bpsbioscience/LSD2(KDM1B)均相分析试剂盒(一甲基)/50613/384反应
商品编号: 50613
品牌: bpsbioscience
市场价: ¥20500.00
美元价: 12300.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description:
TheLSD2(KDM1B)HomogeneousAssayKit(Monomethyl)isdesignedtomeasuretheactivityoflysine-specificdemethylase(LSD2)forscreeningandprofilingapplications.LSD2,alsocalledKDM1B,isachromatin-modifyingenzymethatspecificallyremovesmethylgroupsfrommono-anddimethylatedLysofhistoneH3,therebyactingasacorepressoroftranscription.LSD2isapotentialtargetfordrugdevelopment.TheLSD2HomogeneousAssayKit(Monomethyl)comesinaconvenientAlphaLISA®format,withbiotinylated,mono-methylatedhistoneH3(1MeK4)peptidesubstrate,primaryantibodytodetectnon-methylatedproduct,LSD2assaybuffer,andpurifiedLSD2for384enzymereactions.ThekeytotheLSD2HomogeneousAssayKit(Monomethyl)isahighlyspecificantibodythatrecognizesdemethylatedsubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredfordemethylasedetection.First,asamplecontainingLSD2enzymeisincubatedwiththebiotinylatedsubstrate.Next,acceptorbeadsandprimaryantibodyareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
SuppliedAs:
TheLSD2HomogeneousAssayKit1comesinaconvenientAlphaLISA®format,withbiotinylated,mono-methylatedhistoneH3(1MeK4)peptidesubstrate,primaryantibodytodetectnon-methylatedproduct,LSD2assaybuffer,andpurifiedLSD2.
Contraindications:
GreenandbluedyesthatabsorblightintheAlphaScreensignalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphaScreenassays.
Format:

COMPONENTS:

Storage/StABIlity:
Atleastoneyearfromdateofreceiptwhenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
Binda,C.,etal.,J.Am.Chem.Soc.2010;132(19):6827-6833.

Welte,M.,etal.,CellPress.2005;15(14):1266-1275.

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。