Bpsbioscience/LSD2(KDM1B),GST Tev标签/50124/20µg
								
										商品编号:
										50124
									
									
										品牌:
										bpsbioscience
									
									
								
										市场价:
										¥9507500.00
									
									
										美元价:
										5704500.00
									
								
										联系Q Q:
										3392242852
									
									
										电话号码:
										4000-520-616
									
								
										电子邮箱:
										info@ebiomall.com
									
									商品介绍
						Species:Human
HostSpecies:Sf9insectcells
MW:121kDa
Genbank#:153042
Tag(s):N-terminalGST-TevTag
a.a:2-822
Description:
HumanLSD2protein,alsoknownasKDM1B,GenBankAccessionNo.NM_153042,a.a.2-822withN-terminalGST-Tev-Tag.MW=121kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
Synonym(s):
KDM1B
AssayConditions:
10μLofreactionmixturecontains50mMTris,pH8.8,50mMNaCl,0.01%Tween-20,0.5µMbiotinylatedpeptidesubstrate,andvariousamountofLSD2.Thereactionswereincubatedatroomtemperatureforonehourwithslowshaking.Afterthereaction,10μLofprimaryantibody(1:800dilution)andanti-mouseacceptorbeads(1:500dilution)wasaddedintothewellsandthereactionswereincubatedfor30min.Then10μLofStreptavidin-conjugateddonorbeads(1:125dilution)wasaddedintothewells.Thereactionswereincubatedforadditional30minatroomtemperaturefollowedbyAlphascreeningdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween20,20%glycerol,and1.6mMglutathione
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Binda,C.,etal.,JournalofAmericanChemicalSociety.2010;132(19):6827-6833.
					2.Welte,M.,etal.,CellPress.2005;15(14):1266-1275.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
						bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。
					
		