Bpsbioscience/LSD1（KDM1A），变体2，GST标签/50100/50µg
商品编号：
50100
品牌：
bpsbioscience
市场价：
¥9000.00
美元价：
5400.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:104kDa
Genbank#:NM_015013
Tag(s):N-terminalGST-tag
a.a:158–end
Description:
Variant2HumanLSD1[alsoknownasKDM1A(lysine(K)-specificdemethylase1A)orAOF2,amineoxidase(flavincontaining)domain2)]GenBankAccessionNo.NM_015013,a.a.158-endwithN-terminalGST-tag,MW=103kDa,expressedinanE.coliexpressionsystem.
UniProt
O60341
Synonym(s):
KDM1A,AOF2,lysine(K)-specificdemethylase1A,amineoxidase(flavincontaining)domain2,LSD
SpecificActivity:
≥0.50pmole/min/μg
AssayConditions:
10μlreactionmixcontainingassaybufferwith50mMTrisHClpH8.8,50mMNaCl,1mMDTT,0.01%Tween-20,histoneH3peptidesubstrate,andLSD1(6.7–40ng)addtothewells.Incubatefor1hr.AddantibodyagainstunmethylatedK4peptide.Incubatefor30min,thenStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphaScreeningdetection.
Formulation:
50mMNa2HPO4/NaH2PO4,pH7.4
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Lan,F.etal.,Nature448,7154,718-722,2007.
2.Forneris,F.etal.,J.Biol.Chem.282,28,20070-20074,2007.
ApplicationReference(s):
1.Ascintillationproximityassayforhistonedemethylases(2014)
2.CrystalstructureofthehistonelysinespecificdemethylaseLSD1complexedwithtetrahydrofolate(2014)
Notes:
Important:IfyouintendtoassessLSD1activityinanassaythatanalyzeshydrogenperoxide,suchasBPSCatalog#50106and50107,pleaseuseBPSCatalog50204,LSD1,GST-Tag.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
