Bpsbioscience/JMJD3 (KDM6B), FLAG-tag/50115/20 µg
商品编号：
50115
品牌：
bpsbioscience
市场价：
¥9500.00
美元价：
5700.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:71kDa
Genbank#:NM_001080424
Tag(s):C-terminalFLAG-tag
a.a:1043–end
Description:
HumanJMJD3(KDM6B)histonedemethylase(a.a.1043-end),GenbankAccessionNo.NM_001080424,withC-terminalFLAG-tag,MW=71kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
O15054
Synonym(s):
JMJD3,KDM6B
SpecificActivity:
1.1pmole/min/μg
AssayConditions:
10µlreactionmixcontainingassaybufferwith20mMHEPES(pH7.4),150mMNaCl500µMα-ketoglutarate,25µMiron,2mMascorbicacid,0.01%Tween-20,0.5µMbiotinylatedpeptidesubstrate,andJMJD3(100–500ng)addedtothewells.AddantibodyagainstdemethylatedK27peptide.Incubatefor30min,thenStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphaScreeningdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCland20%Glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Y.Xiang,etal.,CellResearch(2007)17:850–8572.
2.S.Hong,etal.Proc.Natl.Acad.Sci.USA(2007)104(47):18439-44.
3.SwigutT,WysockaJ.Cell(2007)131(1):29-3.
ApplicationReferences:
1.AsmallmoleculemodulatesJumonjihistonedemethylaseactivityandselectivelyinhibitscancergrowth(2013)
2.IdentificationofsmallmoleculeinhibitorsofJumonjiAT-richinteractivedomain1B(JARID1B)histonedemethylasebyasensitivehighthroughputscreen(2013)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
