Bpsbioscience/JMJD2D (KDM4D), GST-tag/50117/100 µg
商品编号：
50117
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9cells
MW:67kDa
Genbank#:NM_018039
Tag(s):N-terminalGST-tag
a.a:2–354
Description:
HumanJMJD2D(GenBankAccessionNo.NM_018039),aminoacids2–354,withN-terminalGSTtag,MW=67kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q6B0I6
Synonym(s):
KDM4D
SpecificActivity:
≥ 0.14pmole/min/μg
AssayConditions:
10µlreactionmixcontainingassaybufferwith20mMHEPES(pH7.4),50mMNaCl,1mMTCEP,500µMα-ketoglutarate,25µMiron,2mMascorbicacid,0.01%Tween-20,0.5µMbiotinylatedpeptidesubstrate,andJMJD2D(5–25ng)addedtothewells.AddantibodyagainstdemethylatedK9peptide.Incubatefor30min,thenStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphaScreeningdetection.
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,3mMDTTand20%glycerol
Storage/StABIlity:
Atleast6monthsat–80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Katoh,M.,IntJOncol.2004Jun;24(6):1623-8.
2.Shin,S.,etal.,BiochemBiophysResCommun.2007Feb23;353(4):973-7.
ApplicationReference(s):
AsmallmoleculemodulatesJumonjihistonedemethylaseactivityandselectivelyinhibitscancergrowth(2013)
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
