Bpsbioscience/JMJD2A(KDM4A) (1-350), His-tag/50123/100 µg
商品编号：
50123
品牌：
bpsbioscience
市场价：
¥9300.00
美元价：
5580.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:42kDa
Genbank#:NM_014663
Tag(s):N-terminalHis-tag
a.a:1–350
Description:
HumanJMJD2A,alsoknownasJHDM3AandKDM4A(GenBankAccessionNo.NM_014663)(a.a.1-350)withN-terminalHis-tag,MW=42kDa,expressedinanE.coliexpressionsystem.
UniProt
O75164
Synonym(s):
JMJD2A,JHDM3A,KDM4A
SpecificActivity:
0.084pmole/min/μg
AssayConditions:
10µlreactionmixcontainingassaybufferwith20mMHEPES(pH7.4),150mMNaCl,500µMα-ketoglutarate,25µMiron,2mMascorbicacid,0.01%Tween-20,0.5µMbiotinylatedpeptidesubstrate,andJMJD2A(100–400ng)addedtothewells.AddantibodyagainstdemethylatedK9peptide.Incubatefor30min,thenStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphaScreeningdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,250mMimidazole,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Zhang,D.,etal.(2005).Mol.CellBiol.25(15):6404-6414.
2.GraySG,etal.(2005).J.Biol.Chem.280(31):28507–18.
ApplicationReference(s):
Ascintillationproximityassayforhistonedemethylases(2014)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
