Bpsbioscience/JMJD1B (KDM3B), FLAG-tag/50421/20 µg
商品编号：
50421
品牌：
bpsbioscience
市场价：
¥9300.00
美元价：
5580.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 195 kDa
Genbank #: NM_016604
Tag(s): N-terminal Avi-, FLAG-tags
a.a: 2-1761 (end)
Description:
Human JMJD1B, also known
as lysine-specific demethylase 3B
(KDM3B), JHDM2B, and KIA1082,
GenBank Accession No. NM_016604/
NP_057688, a.a. 2-1761 (end) with Nterminal
Avi- and FLAG-tags, MW=195
kDa, expressed in a baculovirus-infected
Sf9 cell expression system.
UniProt
Q7LBC6
Synonym(s):
KDM3B, JHDM2B, KIA1082
Specific Activity:
0.0045 pmole/min/μg
Assay Conditions:
Reaction was
performed with 20 mM HEPES (pH 7.4),
150 mM NaCl, 500 μM -ketoglutarate, 25
μM iron, 2 mM ascorbic acid, 0.01%
Tween-20, 0.5 μM biotinylated peptide
substrate, and JMJD1B added to the
wells. Add antibody against methylated
H3K9. Incubate for 30 min. followed by
Alphascreen® detection.
Formulation:
40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 0.04%
Tween-20, 20% glycerol, 80 μg/ml FLAG
peptide
Format:
Aqueous buffer solution
Storage / Stability:
At least 6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Kim, J-Y, et al., Mol. Cel. Biol. 2012. 32(14):2917-2933.
2. Mikhaleva, I.I., et al., Peptides. 2011. 32(4): 826-831.
Warning(s):
Avoid freeze/thaw cycles. Storing diluted
enzyme is not recommended, if
necessary, use carrier protein (BSA 0.1
– 0.5%).
Scientific Category:
Demethylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
