Bpsbioscience/JHDM1D(KDM7A),标志标签/50419/20和micro;G
商品编号:
50419
品牌:
bpsbioscience
市场价:
¥9000.00
美元价:
5400.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9InsectCells
MW:56kDa
Genbank#:NM_030647
Tag(s):N-terminalFLAG-tag
a.a:2-488
Description:
HumanJHDM1D,alsoknownasLysine-specificdemethylase7A(KDM7A)andKIAA1718,GenBankAccessionNo.NM_030647a.a.2-488withanN-terminalFLAG-tag,MW=56kDa,expressedinabaculovirus-infectedSf9cellexpressionsystem.
UniProt
A0JNV9
Synonym(s):
Lysine-specificdemethylase7A,KDM7A,KIAA1718
SpecificActivity:
0.2pmole/min/μg
AssayConditions:
AssayConditions:10μlreactionmixcontainingassaybufferwith20mMHEPES(pH7.4),50mMNaCl,1mMTCEP,500μMalpha-ketoglutarate,25μMiron,2mMascorbicacid,0.01%Tween-20,0.5μMbiotinylatedpeptidesubstrate,andJHDM1D(8–40ng)addedtothewells.AddantibodyagainstmonomethylatedK9peptide.Incubatefor30min,thenStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphaScreeningdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,20%glycerol,80μg/mlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Yang,C.,etal.CellRes.2010.20:886-898.
2.Osawa,T.etal.Proc.Nat.Acad.Sci.(USA).2011.108(51):20725-20729.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。