Bpsbioscience/JARID1B（KDM5B），全长，标志标签/50128/20µ；G
商品编号：
50128
品牌：
bpsbioscience
市场价：
¥9000.00
美元价：
5400.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:179kDa
Genbank#:NM_006618
Tag(s):N-terminalFLAG-Avi-tag
a.a:2-1544(end)
Description:
HumanJARID1B,alsoknownasKDM5BandPLU-1(GenBankAccessionNo.NM_006618),a.a.2-1544(end)withN-terminalFLAG-Avi-tag,MW=179kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
Q9UGL1
Synonym(s):
JARID1B,PLU-1,KDM5B,Retinoblastoma-BindingProtein2Homolog1,RBP2-H1,CT31,Cancer/TestisAntigen31,PLU-1,Jumonji,ATRichInteractiveDomain1B,Lysine-SpecificDemethylase5B
SpecificActivity:
0.11pmol/min/µg
AssayConditions:
10µlreactionmixcontaining20mMHEPES(pH7.4),50mMNaCl,500µMα-ketoglutarate,25µMiron,2mMascorbicacid,0.01%Tween-20,1mMTCEP,0.5µMbiotinylatedpeptidesubstrate,andJARID1B.Incubate1houratRTwithslowshaking.Addantibodyagainstdemethylatedproductandanti-mouseacceptorbeads.After30minutes,addStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphascreeningdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,20%glycerol,80μg/mlFLAGpeptide,and0.2mMTCEP
Storage/StABIlity:
Atleast6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.YamaneK,etal.(2007).Mol.Cell25(6):801–12.
2.BarrettA,etal.(2007).Int.J.Cancer121(2):265–75.
2.BarrettA,etal.(2007).Int.J.Cancer121(2):265–75.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Demethylases
ProductType:
Enzymes
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
