Bpsbioscience/JARID1B（KDM5B）TR-FRET分析试剂盒/50514/384反应
商品编号：
50514
品牌：
bpsbioscience
市场价：
¥41100.00
美元价：
24660.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
JARID1B,alsoknownasPLU-1andKDM5B,isaJumonjiC(JmjC)andARIDdomain-containinghistonelysinedemethylasethatexhibitsdemethylationactivitytowarddi-andtrimethyl-lysine4(H3K4me2/3)onhistoneH3.
Description:
TheJARID1BTR-FRETAssayKitisdesignedtomeasureactivityofJARID1Binahomogeneous384reactionformat.ThisFRET-basedassayrequiresnotime-consumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.TheJARID1BTR-FRETAssayKitcomesinaconvenientformat,withhistoneH3peptidesubstrate,aEu-labeledantibodyagainstmethylatedK4residueofHistoneH3,demethylaseassaybuffer,TR-FRETdetectionbuffer,dye-labeledacceptor,andpurifiedJARID1Bfor384enzymereactions.ThekeytotheJARID1BAssayKitisahighlyspecificantibodythatrecognizesdemethylatedsubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredfordemethylaseactivitydetection.First,asamplecontainingJARID1Benzymeisincubatedwiththebiotinylatedsubstrate.Next,antibodyisadded.Finally,dye-labeledacceptorisaddedfollowedbyfluorescencereADIng.
Synonym(s):
JARID1B,PLU-1,KDM5B
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleast6monthsfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Lahoud,M.H.,etal.GenomeRes.11(8):1327–34.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescenceResonanceEnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescenceResonanceEnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
