Bpsbioscience/JARID1A (KDM5A), FLAG-tag/50110/20 µg
商品编号:
50110
品牌:
bpsbioscience
市场价:
¥9500.00
美元价:
5700.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:125kDa
Genbank#:NM_001042603
Tag(s):C-terminalFLAG-tag
a.a:1–1090
Description:
HumanJARID1A,alsoknownasKDM5AandRBBP2,GenBankAccession#NM_001042603),a.a.1-1090withC-terminalFLAG-tag,MW=125kDa,expressedinSf9cellsviaabaculovirusexpressionsystem.
UniProt
P29375
Synonym(s):
KDM5A,RBBP2,JARID1A
SpecificActivity:
0.05pmol/min/µg
AssayConditions:
10µlreactionmixcontainingassaybufferwith20mMHEPES(pH7.4),50mMNaCl,1mMTCEP,500µMalpha-ketoglutarate,25µMiron,2mMascorbicacid,0.01%Tween-20,0.5µMbiotinylatedpeptidesubstrate,andJarid1A(20–100ng)addedtothewells.Addantibodyagainstdi-methylatedK4peptide.Incubatefor30min,thenStreptavidin-conjugatedsecondaryantibodyfollowedbyAlphaScreeningdetection.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and80µg/mlFLAGpeptide
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat–80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Roesch,A.,etal.,(2005).ModPathol.18(9):1249-57.
2.Zhou,X.,etal.,(2010).CancerRes.70(10):4214-21
3.VanZutven,LJ.,etal.,(2006).GenesChromosomesCancer45(5):437-46.
ApplicationReference(s):
AsmallmoleculemodulatesJumonjihistonedemethylaseactivityandselectivelyinhibitscancergrowth(2013)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Demethylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。