Bpsbioscience/Sirtuin 1(193-741),GST标签/50012/100µg
商品编号:
50012
品牌:
bpsbioscience
市场价:
¥8800.00
美元价:
5280.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:87.2kDa
Genbank#:NM_012238
Tag(s):N-terminalGST-tag
a.a:193–741
Description:
HumanSirtuin1,GenBankAccessionNo.NM_012238,a.a.193-741withN-terminalGST-tag,MW=87.2kDa,expressedinanE.coliexpressionsystem.
UniProt
Q96EB6
Synonym(s):
Sirtuin1,SIRT1,Sir2,Sir2a
SpecificActivity:
≥20pmol/min/µg
AssayConditions:
25mMTris/HCl,pH8.0,137mMNaCl,2.7mMKCl,1mMMgCl2,and0.1mg/mlBSA,20µMBPSHDACsubstrate1(Catalog#50032),500µMNAD+andSirtuin1(12.5-800ng).Incubationcondition:30minat37°Cfollowedbytreatmentwith50µlofSIRTdeveloper(Catalog#50089)for15minatRT.Fluorescenceintensityismeasuredatex360/em460.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Kolthur-Seetharam,U.etal(2006)CellCycle5,873-877.
2.Solomon,J.M.etal(2006)Mol.Cell.Biol.26,28-38.
ApplicationReference(s):
1.SIRT4IsaLysineDeacylasethatControlsLeucineMetabolismandInsulinSecretion (2017)
2. LysineDeacetylasesExhibitDistinctChangesinActivityProfilesDuetoFluorophoreConjugationofSubstrates(2017)
3.DevelopmentofHigh-ThroughputAssaystoStudyMethylases,DemethylasesandDeacetylases(2011)
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1-0.5%).
ScientificCategory:
Deacetylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。