Bpsbioscience/HDAC6, FLAG-tag/50056/50 µg
商品编号：
50056
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:132kDa
Genbank#:NM_006044
Tag(s):C-terminalFLAG-tag
a.a:fulllength
Description:
HumanHDAC6(GenBankAccessionNo.NM_006044),fulllengthwithC-terminalFLAG-tag,MW=132kDa,expressedinbaculovirusexpressionsystem.
UniProt
Q9UBN7
Synonym(s):
histonedeacetylase6,HDAC-6
SpecificActivity:
≥146pmol/min/µg.
UnitDefinition:
OneU=1pmolofacetylgroupremoved/min/µgofenzyme
AssayConditions:
25mMTris/HCl,pH8.0,137mMNaCl,2.7mMKCl,1mMMgCl2,and0.1mg/mlBSA,30µMBPSHDACsubstrate(Catalog#50037),andHDAC6.Incubationcondition:30minat37°CfollowedbyHDACdeveloper(Catalog#50030)for15minatroomtemperature.Fluorescenceintensityismeasuredatex360/em460.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,80ng/µlFLAGpeptide,and20%glycerol.
Purification:
Proteinwaspurifiedbyaffinitychromatographyandgelfiltration.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
Strausberg,R.L.etal.,Proc.Natl.Acad.Sci.U.S.A.99(26),16899-16903(2002).
ApplicationReferences:
1.Kineticmethodforthelarge-scaleanalysisofthebindingmechanismofhistonedeacetylaseinhibitors(2014)
2.Potenthistonedeacetylaseinhibitorsderivedfrom4-(aminomethyl)-N-hydroxybenzamidewithhighselectivityfortheHDAC6isoform(2013)
3.SantacruzamateA,apotentandselectivehistonedeacetylaseinhibitorfromthePanamanianmarinecyanobacteriumcf.Symplocasp(2013)
4.ThioesterderivativesofthenaturalproductpsammaplinAaspotenthistonedeacetylaseinhibitors(2013)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
