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当前位置: 首页 > 产品中心 > cytokine > Bpsbioscience/HDAC5,催化结构域,His标签/50005/10µg
商品详细Bpsbioscience/HDAC5,催化结构域,His标签/50005/10µg
Bpsbioscience/HDAC5,催化结构域,His标签/50005/10µg
Bpsbioscience/HDAC5,催化结构域,His标签/50005/10µg
商品编号: 50005
品牌: bpsbioscience
市场价: ¥8800.00
美元价: 5280.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞因子
公司分类: cytokine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 51 kDa
Genbank #: NM_005474
Tag(s): C-terminal His-tag
a.a: 656–1122 (end)
Description:
Human HDAC5 catalytic domain , GenBank Accession No. NM_005474, a.a. 656-1122 (end) with C-terminal His-tag, MW= 51 kDa, expressed in baculovirus expression system.
UniProt
Q9UQL6
Synonym(s):
histone deacetylase 5, HDAC-5
Specific Activity:
≥1000 pmol/min/µg.
Unit Definition:
One U =1 pmol of acetyl group removed/min/µg of enzyme
Assay Conditions:
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 µM BPS HDAC class 2a substrate , BPS Catalog number 50040, and HDAC5. Incubation condition: 30 min at 30°C, followed by HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
Formulation:
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 imidazole, and 10% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-41776
2. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.

Application References:
1. An azumamide C analogue without the zinc-binding functionality (2017)
2. Impact of binding mechanism on selective inhibition of histone deacetylase isoforms (2017)
3. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
4. Identification and characterization of small-molecule inhibitors of lysine acetyltransferases (2014)
5. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
6. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
7. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
8. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
9. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。