Bpsbioscience/HDAC11荧光检测试剂盒/50687/96反应-免疫在线蚂蚁淘旗下平台

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商品详细Bpsbioscience/HDAC11荧光检测试剂盒/50687/96反应

Bpsbioscience/HDAC11荧光检测试剂盒/50687/96反应

商品编号： 50687

品牌： bpsbioscience

市场价： ¥8900.00

美元价： 5340.00

产地：美国(厂家直采)

公司：

产品分类：其它检测试剂盒

公司分类： Other_kits

联系Q Q： 3392242852

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商品介绍

Description:

TheHDAC11FluorogenicAssayKitisacompleteassaysystemdesignedtomeasurehistonedeacetylase11(HDAC11)activityforscreeningandprofilingapplications.TheHDAC11FluorogenicAssayKitisbasedonauniquefluorogenicsubstrateanddevelopercombination.ThisassaymethodeliminatesdealingwiththerADIoactivity,extraction,andchromatographyaspectsoftraditionalassays.Usingthiskit,onlytwosimplestepsonamicrotiterplateareneededtoanalyzetheHDAC11activitylevel.First,theHDACfluorometricsubstrate,containinganacetylatedlysinesidechain,isincubatedwithpurifiedHDAC11.ThedeacetylationsensitizesthesubstratesosubsequenttreatmentwiththeLysineDeveloperproducesafluorophorethatcanthenbemeasuredusingafluorescencereader.

HDACsregulatecellularprocessesbycatalyzingthehydrolysisofanacetylgroupfromacetyllysinesinmodifiedproteins.IntheHDACassay,fluorescent-dyemoleculesareattachedtoapeptidecontainingacetyllysine.Attachmenttothepeptidequenchesthefluorescenceofthedye.AftertreatmentofthepeptidewithanHDAC,thereactionismixedwithadevelopmentsolutionthatisspecificfornonacetylatedlysines.IftheacetylgrouphasbeenremovedfromthelysinebytheHDAC,thissolutionwillreleasethedyeallowingforfluorescence.TheamountoffluorescenceisthereforedirectlyrelatedtothelevelofHDACactivity.

Synonym(s):

histonedeacetylase11,HDAC-11

SuppliedAs:

Itcomesinaconvenient96-wellformat,withallthereagentsnecessaryfor100fluorescentHDAC11activitymeasurements.Inaddition,thekitincludespurifiedHDAC11enzymeandapotentHDACinhibitor,TrichostatinA,foruseasapositiveandnegative

Format:

SBIoscience.com/media/wysiwyg/50687.JPG"

Instructionsforuse:

Seeassaykitdatasheetfordetailedprotocol.

Storage/StABIlity:

Stableatleastoneyearfromdateofreceiptwhenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions,Besuretostoreeachcomponentatthepropertemperatureuponarrival.

Application(s):

GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.

Reference(s):

Deubzer,H.E.,etal.,Int.J.Cancer.2013May1;132(9):2200-8.

Warning(s):

Avoidfreeze/thawcycles.

ScientificCategory:

HDAC

ProductType:

AssayKit

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

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