Bpsbioscience/HDAC10, GST-tag, His-tag/50010/50 µg
商品编号：
50010
品牌：
bpsbioscience
市场价：
¥8200.00
美元价：
4920.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:78kDa
Genbank#:NM_032019
Tag(s):N-terminalGST-tag,C-terminalHis-tag
a.a:1–481
Description:
HumanHDAC10(a.a.1-481),GenBankAccessionNo.NM_032019,withN-terminalGST-tagamdC-terminalHis-tag,MW=78kDa,expressedinbaculovirusexpressionsystem.
UniProt
Q969S8
Synonym(s):
histonedeacetylase10,HDAC10
SpecificActivity:
≥4.4pmol/min/µg.
AssayConditions:
25mMTrisHCl,pH8.0,137mMNaCl,2.7mMKCl,1mMMgCl2,and0.1mg/mlBSA,20µMBPSHDACsubstrate3(Catalog#50037),andHDAC10(15.6-2000ng).Incubationcondition:30minat37°C,followedbyHDACdeveloper(Catalog#50030)for15minatroomtemperature.Fluorescenceintensityismeasuredatex360/em460.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.ChakrabortyS.(2006)J.Biol.Chem.281(46):35070-80.
2.Wolfgang,F.(2001)J.Biol.Chem.276(38):35826-35835.
ApplicationReferences:
1.AnazumamideCanaloguewithoutthezinc-bindingfunctionality(2017)
2. Suppressionofmonosodiumuratecrystal-inducedcytokineproductionbybutyrateismediatedbytheinhibitionofclassIhistonedeacetylases(2015)
3.Design,synthesis,3Dpharmacophore,QSAR,anddockingstudiesofcarboxylicacidderivativesasHistoneDeacetylaseinhibitorsandcytotoxicagents(2014)
4.Theeffectofvariouszincbindinggroupsoninhibitionofhistonedeacetylases1-11(2014)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Deacetylase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
