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当前位置: 首页 > 产品中心 > cytokine > Bpsbioscience/HDAC1,标志标签,His标签/50051/50µg
商品详细Bpsbioscience/HDAC1,标志标签,His标签/50051/50µg
Bpsbioscience/HDAC1,标志标签,His标签/50051/50µg
Bpsbioscience/HDAC1,标志标签,His标签/50051/50µg
商品编号: 50051
品牌: bpsbioscience
市场价: ¥8800.00
美元价: 5280.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞因子
公司分类: cytokine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 56 kDa
Genbank #: NM_004964
Tag(s): C-terminal FLAG-tag, C-terminal His-tag
a.a: full length
Description:
Human HDAC1 protein (GenBank Accession No. NM_004964), full length with C-terminal His-tag and C-terminal FLAG-tag, MW = 56 kDa, expressed in baculovirus expression system. Recombinant HDAC1 forms a complex with endogeneous Hsp70 and is co-purified with tubulin. The identity of Hsp70 and tubulin was confirmed by MALDITOF mass spectrometry.
UniProt
Q13547
Synonym(s):
histone deacetylase 1, HDAC-1, HDAC1 Tag
Specific Activity:
≥460 pmol/min/µg.
Assay Conditions:
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC substrate 3 (Catalog #50037), and HDAC1. HDAC reaction was done for 30 min at 37°C followed by treatment with 50 µl of HDAC developer (Cat. #50030) for 15 min at RT. Fluorescence intensity is measured at ex360/em460.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide, and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:

>6 months at -80°C.

Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):

1. Rikimaru,T. et al., Oncology 72 (1-2), 69-74 (2007).
2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).

Application Reference(s):
1. Structure-Activity Relationship of Propargylamine-Based HDAC Inhibitors (2017)
2. HDAC Inhibitor-Induced Mitotic Arrest Is Mediated by Eg5/KIF11 Acetylation (2017)
3. Screening of histone deacetylase 1 inhibitors in natural products by capillary electrophoresis (2017)
4. (±)-Applanatumines B–D: novel dimeric meroterpenoids from Ganoderma applanatum as inhibitors of JAK3 (2017)
5. Synthese, Konformationsanalyse und biologische Evaluierung von neuartigen antitumoraktiven Peptoid-Derivaten (2017)
6. Alkoxyurea-Based Histone Deacetylase Inhibitors Increase Cisplatin Potency in Chemoresistant Cancer Cell Lines (2017)
7. Discovery of an Inhibitor of the Proteasome Subunit Rpn11 (2017)
8. Dipicolinic Acid Derivatives as Inhibitors of New Delhi Metallo-β-lactamase-1  (2017)
9. Divergent JNK Phosphorylation of HDAC3 in Triple-Negative Breast Cancer Cells Determines HDAC Inhibitor Binding and Selectivity (2017)
10. Synthesis and Biochemical Evaluation of Biotinylated Conjugates of Largazole Analogues: Selective Class I Histone Deacetylase Inhibitors (2017)
11. An azumamide C analogue without the zinc-binding functionality (2017)
12. Impact of binding mechanism on selective inhibition of histone deacetylase isoforms (2017)
13. Structure-based design and biological characterization of selective histone deacetylase 8 (HDAC8) inhibitors with anti-neuroblastoma activity (2017)
14. Design, synthesis and biological evaluation of novel hydroxamic acid based histone deacetylase 6 selective inhibitors bearing phenylpyrazol scaffold as surface recognition motif (2017)
15. Identification of histone deacetylase inhibitors with benzoylhydrazide scaffold that selectively inhibit class I histone deacetylases (2015)
16. A chimeric SERM-histone deacetylase inhibitor approach to breast cancer therapy (2014)
17. CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis (2014)
18. Design, synthesis and evaluation of novel HDAC inhibitors as potential antitumor agents (2014)
19. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014)
20. Discovery of a small molecule agonist of phosphatidylinositol 3-kinase p110α that reactivates latent HIV-1 (2014)
21. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014)
22. Kinetic method for the large-scale analysis of the binding mechanism of histone deacetylase inhibitors (2014)
23. Selective HDAC Inhibition for the Disruption of Latent HIV-1 Infection (2014)
24. The effect of various zinc binding groups on inhibition of histone deacetylases 1-11 (2014)
25. Thermodynamics of ligand binding to histone deacetylase like amidohydrolase from Bordetella/Alcaligenes (2014)
26. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013)
27. Class I HDAC imaging using [ (3)H]CI-994 autoradiography (2013)
28. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013)
29. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
30. Highly ligand efficient and selective N-2-(Thioethyl)picolinamide histone deacetylase inhibitors inspired by the natural product psammaplin A (2013)
31. Imaging epigenetic regulation by histone deacetylases in the brain using PET/MRI with ¹⁸F-FAHA (2013)
32. Structural basis for the inhibition of histone deacetylase 8 (HDAC8), a key epigenetic player in the blood fluke Schistosoma mansoni (2013)
33. Thioester derivatives of the natural product psammaplin A as potent histone deacetylase inhibitors (2013

Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Deacetylase
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。