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当前位置: 首页 > 产品中心 > cytokine > Bpsbioscience/Fluorogenic Sirtuin 5 Substrate/50126/250 nmole
商品详细Bpsbioscience/Fluorogenic Sirtuin 5 Substrate/50126/250 nmole
Bpsbioscience/Fluorogenic Sirtuin 5 Substrate/50126/250 nmole
Bpsbioscience/Fluorogenic Sirtuin 5 Substrate/50126/250 nmole
商品编号: 50126
品牌: bpsbioscience
市场价: ¥4700.00
美元价: 2820.00
产地: 美国(厂家直采)
公司:
产品分类: 细胞因子
公司分类: cytokine
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description:
Afluorogenic,succinylatedpeptidesubstrateforSirtuin5.ThissubstrateshouldbeusedinconjunctionwithSIRTDeveloper,#50089.Optimalexcitationwavelength350-380nm,emissionwavelength440-460nm.
UniProt
Q9NXA8
Synonym(s):
SIRT5
Purity:
>90%byHPLC
SuppliedAs:
DMSOsolution(50µl@5mM)
Format:
DMSOsolution
Instructionsforuse:

ASSAYPROTOCOL:

Materials:SIRTAssayBuffer(BPS#50090)SIRT5Substrate(#50126)SIRT5enzyme(#50016)orotherSIRT5-containingsampleNUNCblack96-wellplate,lowproteinbinding(VWR#62408-936).SIRTAssayDeveloper(BPS#50089)

Step1:Setup50μlofSirt5reactionscontainingSIRTassaybuffer,400ngSIRT5enzyme,and20μMSIRT5substrateinthe96-wellplate.Incubateat37ºCfor30min.

Step2:Add50μlofSIRTAssayDeveloper(2x)(BPS#50089)toeachwellandincubatetheplateatroomtemperaturefor15min.

Step3:Readsampleinamicrotiter-platereADIngfluorimetercapableofexcitationatawavelengthintherange350-380nmanddetectionofemittedlightintherange440-460nm.“Blank”value(noenzymecontrol)issubtractedfromallothervalues.

Storage/StABIlity:

Stableatleast6monthsfromdateofreceipt,whenstoredasdirectedat-20°Cor-80°C

Application(s):
SuitableforusewithBPS’sFluorogenicSIRT5AssayKit,#50085,andtostudyenzymekineticsandscreensmallmoleculeinhibitorsofSirtuin5fordrugdiscoveryandHTSapplications.
Reference(s):

1.Madsen,A.S.andOlsen,C.A.,J.Med.Chem.2012,55:5582-5590.
2.Du,J.,etal.,Science,11November2011,334:806-809.

Warning(s):
Avoidfreeze/thawcycles.Protectfromlight.
ScientificCategory:
Deacetylase
ProductType:
Substrate
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。